- City of Darwin and Anchorage, Alaska, U.S.A.
- City of Darwin and Haikou City, Hainan Province of the People’s Republic of China
- City of Darwin and Milikapiti Community Government Council, N.T.
- City of Darwin and Kalymnos, Greece
- City of Darwin and Dili, East Timor
- City of Darwin and Ambon, Maluku, Republic of Indonesia
- City of Darwin and Honolulu, USA
- City of Darwin and Gobierno Municipal de Santa Cruz-Galapagos, Ecuador
- City of Darwin and Guangzhou, the People’s Republic of China
- City of Darwin and Qingdao City Government, the People’s Republic of China
- City of Darwin and Yuexiu District, Guangzhou, Guangdong, People’s Republic of China